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Let them dissolve and throw it all back
The structural diversity of these compounds is attributed to their biosynthetic origins, including organosulfur compounds from Allium species, glucosinolates from cruciferous vegetables, and other sulfur metabolites from microbial and marine sources
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The cell pellet was quickly resuspended in a hypotonic lysis buffer (containing 10 mM HEPES (H109408-5g, Aladdin, Shanghai, China), pH 7.9, 1.5 mM MgCl2 (M434105-100g, Aladdin, Shanghai, China), 10 mM KCl (P433501-250g, Aladdin, Shanghai, China), 0.5 mM DTT (D301909-1ml, Aladdin, Shanghai, China), and washed with it