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Louis, MO), The lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

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Despite these promising findings, further investigation is warranted to fully understand the therapeutic effects and mechanisms of lowdose IL2 in treating AA due to limited available data