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The DCF was quantified using a standard curve as previously reported ( 2.7 Malondialdehyde (MDA) lipid peroxidation assay 200 L of cytosol samples were mixed with 1 mL of acetic acid (20%) then 200 L of 8% sodium dodecyl sulphate

Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

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The mixture was ground again for 60 s at 50 Hz, and then centrifuged at 4 C for 10 min at 12,000 rpm

Other strains Other strains of probiotics may also help reduce weight and belly fat