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The wide inter-individual variability in disease pattern favours a switch-over trial design which, however, is foiled by the carry-over effect of todays high potency material eg

Following permeabilization, the blastocysts were placed in a solution containing propidium iodide (for 30 s, PI, P4170, Sigma-Aldrich, 100 g/ml) and then washed in PBS-PVA before being transferred to a solution containing 4% paraformaldehyde (158127, Sigma-Aldrich, w/v) + Hoechst 33,342 (for 15 min, B2261, Sigma-Aldrich, 25 g/ml)

doi: 10.1016/j.bcp.2007.04.010 103

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