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Description

Conjugated reporter enzyme is typically horseradish peroxidase (HRP) or alkaline phosphatase (AP) Note: This step may be omitted if the reporter enzyme is directly conjugated to the detection antibody Add substrate and incubate to develop signal Measure signal in a plate reader Competitive ELISA kit In competitive enzyme immunoassays (EIA) the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a reporter enzyme

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