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This dual pathway activation allows for investigation of potential synergistic effects between GHRH and ghrelin signaling systems, providing opportunities to study how these complementary mechanisms may work together to optimize growth hormone release patterns

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Immunohistochemistry and advanced imaging techniques Tissue sections underwent immunohistochemical staining using primary antibodies (CD68, F4/80, Ki67, PCNA, CD31, VEGFR2, CK-18, -Tubulin, Mucin5AC, Aggrecan, COL2