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The basal ROS production, which accounts for approximately 2% of total mitochondrial activity even under optimal conditions, underscores their essential role in cellular physiology (Figure 1) [3, 5]

Our results showed that 30-day intermittent fasting from dawn to sunset, the human activity phase, was associated with an anticancer serum proteome response and upregulated several key regulatory proteins that play a key role in tumor suppression, DNA repair, insulin signaling, glucose, and lipid metabolism, circadian clock, cytoskeletal remodeling, immune system, and cognitive function 15

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It is also depleted by oxidative stress caused by free radicals, since it is used up while neutralizing these unstable molecules

doi: 10.15252/embj.2019101982 85 WaaijerMECGunnDAAdamsPDPawlikowskiJSGriffithsCEMvan HeemstDet al