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The tissue pieces for immunohistochemistry were deparaffinized and rehydrated, then incubated with smooth muscle actin (-SMA) antibody (Boster, Wuhan, China) for overnight, subsequent 2 h incubation with goat anti-mice IgG

B12 isnt just a nice to have vitamin it is crucial for ensuring the correct function of blood cells, nerves, and the brain

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Common injection sites include: Lower abdomen Thigh area Near the site of injury (for targeted recovery) This route is often favored for tendon or ligament healing