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In the univariate Cox regression model, first line therapy (hazard ratio [HR]=0.393 [95% CI: 0.159-0.974], p=0.044) and baseline sBTLA120 pg/mL (HR=0.237 [95% CI: 0.085-0.660], p=0.006) were significant prognostic factors relevant to progression free survivial (PFS)

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Flies were then washed (twice for 1 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and were reacted with 2.5 mM Ce-DAB2 solution in cacodylate buffer 36 for 75 min to produce the desired brown intensity color from the precipitate

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It is noteworthy that long-term HFD consumption may differentially regulate NF-B activity in hepatocytes (inhibition) and Kupffer cells (activation by lipopolysaccharide)