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42 These findings on the upregulation of the enzymatic machinery involved in PG, LTA, and D-alanine synthesis in conjunction with the downregulation of enzymes implicated in cell wall degradation in biofilm cells, raised the question of whether there is an alteration in the cell wall thickness of biofilm-associated cells compared to floating cells

They start with 510 shots over a few weeks, then gradually space them farther apart, sometimes by months

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