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The researchers then used conjugated dienes and Schiffs bases to assess the intensity of the lipid peroxidase activity while the level of oxidative modification of proteins was assessed by observing the amino acid carbonyl derivative content in proteins following reaction with 2,4-dinitrophenylhydrazine [3]

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In a first MSA-DH in vitro assay, substrate-dependent formation of NADH was monitored spectroscopically (Figure 5A)