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61 However, an investigation of coronary artery occlusion in 23 mongrel dogs administered NAC 30 minutes before and continued until 3 hours after reperfusion failed to observe an increase in total GSH or GSH peroxidase activity at the biopsied ischemic zone, or a decrease in myocyte death

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In this final optimized RP-HPLC method all the compounds of interest separated well in 30 minutes, followed by a re-equilibration to the initial condition