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Sigma-Aldrich, MO, USA) solution for 15 min, at 4C, followed by two washes with 1PBS

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On the other hand, evidence indicates that estradiol also plays an important role in differentiating the human brain during early prenatal stages and even after birth ( The disruption of estrogens homeostasis is involved in the development or progression of a series of pathologies, which include several types of cancer (breast, ovary, colorectal, prostate, endometrium), osteoporosis, autoimmune diseases, cardiovascular diseases, obesity and/or insulin resistance (Patel et al., 2018), as well as neurodegenerative diseases (Villa et al., 2016) and mood disorders (Payne, 2003)