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doi:10.1007/s10522-025-10315-x PMC:PMC12411320 (note: a correction was published 15 November 2025, doi:10.1007/s10522-025-10326-8, replacing Figs

Key points: Lily Bratchell froze her eggs during high school after losing her right ovary to a complex cyst Cysts can cause severe pain when they rupture or twist the ovary Ovarian cysts can affect females of all ages, from young girls to post-menopausal women "I just wanted to roll up into a little ball, I couldn't stand up straight at all because it hurt too much, I was in that much pain," she said

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The entire explant is imaged for all antibody and nuclear labeling in a 3-dimensional X-Y-Z plane in which the Z-plane consists of a stack imaged at intervals of 0.5-5 m