Calcium additives are a common cause of constipation
Molecular docking As no experimentally resolved structure was available, the amino acid sequence of mouse PCBP1 (GeneID: 23983) was modeled using ProteinX, an open-source AlphaFold-like implementation, and the resulting structure was refined with Rosetta Relax to improve stereochemical quality and stability
Animals were transferred to a new plate every 24 h, after which the number of eggs laid and hatched larvae was scored
Differences between groups were analyzed using a Students t test (two-group comparisons) or one-way analysis of variance (multiple-group comparisons) with Tukeys post-hoc multiple comparisons test
This can include nutrients such as iron, calcium, magnesium, zinc, and vitamins A, D, E, K, and B