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Results Glutamate addition reduce opacity in galactose-induced cataracts in rats In the past, pyruvate has been reported to prevent diabetic cataracts in animal studies 20,21

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Aroor A, McKarns S, Nistala R, Demarco V, Gardner M, Garcia-Touza M, Whaley-Connell A, Sowers JR

doi:10.2147/jir.s470520

The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C