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The reaction flask was sealed, covered by aluminum foil to prevent the light, taken out from the glovebox, and stirred at 40 C in an oil bath for 24 h

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Y., Ban, S

ACTL6A impacts GSH de novo synthesis mainly by upregulating -glutamyl-cysteine synthesis To further assess the mechanism by which ACTL6A regulates GSH metabolism, we investigated the metabolic fate of U- 13 C glucose by liquid chromatography with mass spectrometry (LCMS), which produces -GC containing two 13 C atoms via GSH de novo synthesis pathway, GSH containing two 13 C atoms via GSH de novo synthesis or the SGOC pathway, or four 13 C atoms via both pathways (Fig

Interestingly, no correlation occurred between virus titers and defense gene expression in systemic leaves, suggesting that these plant responses are directed primarily against oxidative stress rather than against the invading virus