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The separation was carried out on a Welch Ultisil Amino Acid (4.6250 mm, 5 m) column under a gradient program using sodium acetate trihydrate solution: acetonitrile (93:7) as mobile phase A and acetonitrile: water (80:20) as mobile phase B

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If considering a dose reduction for maintenance: Wait until weight has stabilized for at least 4 weeks at your current dose Reduce by one dose step (e.g., 12mg to 8mg), do not jump multiple steps Monitor weight closely for 8 weeks after the reduction If weight begins to increase, return to the previous maintenance dose The phase 3 trials specifically tested 4mg as a maintenance dose, suggesting Lilly sees viability in lower maintenance doses What happens if you stop Weight regain after discontinuing GLP-1 agonists is well documented with semaglutide and tirzepatide