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However, skin-based changes are not immediately visible

10% fetal bovine serum/FBS (Life Technologies 10270106) and 1% penicillin/streptomycin solution (P/S, Sigma-Aldrich P0781) were used for cell culture, except for DHODH knockout cells, which were cultured with the additional supplement of 50 M uridine at 37 C in a humidified incubator containing 5% CO 2

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The ISSNs 2023 position stand on EAA supplementation (Ferrando et al., 2023) makes the case directly: EAA requirements increase during caloric deficits , free-form EAAs stimulate MPS more effectively than equivalent intact protein, and doses as low as 1.53 g can trigger a synthesis response at rest