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Loose dentures are a common issue among elderly patients, often resulting from natural changes in the mouth over time

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The mechanism and timeline are the same as for male patients, with some adjustments in the protocol for patients in perimenopause or menopause where hormonal changes affect collagen synthesis and healing rates

After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)

Dietary sources of Vitamin B12 include animal products like meat, poultry, fish, dairy, and oral supplements