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Wild-type and mutant constructs were transfected into HEK-293T cells using FuGENE HD (Promega, Madison, WI), with 3 g of DNA and 9 l of the transfection reagent, according to the manufacturers protocol

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Ehrhardt N, Cui J, Dagdeviren S, Saengnipanthkul S, Goodridge HS, Kim JK, et al

*Correspondence: Ming Li, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers