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The cells were then washed (five times for 2 min each) with 0.1 M sodium cacodylate buffer pH 7.4 on ice and were postfixed with 1% osmium tetroxide (EMS, 19150) in 0.1 M sodium cacodylate buffer pH 7.4 for 30 min on ice
Note: if you wake up a bit groggy from the melatonin, try to get some natural light exposure to help rid your body of the melatonin. Oh, and you can also cut the bullets in half to cut down the dosage next time
Henceforth we systematically measured eryptosis phenotypes of cells cultured in iRPMI (without Albumax) at 4% hematocrit and exposed to a 4 h drug incubation period
Ripani et al
PMID: 19106640
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