Detection of cellular thiols was done using monobromobimane (MBB, ThermoFisher M1378) added 10 min before acquisition at 50 M
Distal colon content (200 mg) from two mice for one experiment was homogenized in PBS (1 mL) and centrifuged at 9000 g , the supernatant was collected, and then the low-molecular-weight fraction of the supernatant was fractionated using Amicon Ultra (Ultracel 3 K, Merck, Darmstadt, Germany) and the high-molecular-weight fraction was fractionated using a PD SpinTrap G-25 column (GE Healthcare, Buckinghamshire, UK)
Drug interactions remain largely uncharacterized
The gastrointestinal effects of Mounjaro may also influence ice cream tolerance
GLP-1R agonists (GLP-1RAs) have many functions, such as neuroprotection, inhibiting infection, and metabolic regulation, and show good application prospects in improving cognitive function