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First, each flanking region was amplified from ATCC46645 genomic DNA using primer ogliT1 and ogliT4 for flanking region A (1.3 kb), and ogliT-2 and ogliT-3 for flanking region B (1.2 kb)

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30 As a canonical byproduct of glucose metabolism, it is primarily generated through glycolysis, where glucose is converted to pyruvate and then to lactate by lactate dehydrogenase (LDH)

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