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Waters, Milford, MA) were equilibrated with 6 mL methanol and 2 mL H 2 O before samples were loaded onto columns

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These include the receptor for platelet-activating factor (PAFR) (360-362), prostaglandin receptor EP2 (363,364), and sphingosine-1-phosphate receptor 1 (S1P1) (365)

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To this end, we adapted Grx1roGFP2 for expression in these compartments, and assessed the recovery of the respective local E GSH after oxidative challenge and subsequent removal of the oxidant