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(As expected, the unsaturated fatty acid oleate did not cause ER stress.) Palmitate is thought to elicit ER not by disrupting protein folding per se but by altering ER membrane fluidity, activating the UPR stress sensors through their transmembrane domains (Volmer, van der Ploeg, & Ron, 2013)

doi: 10.1371/journal.pbio.3001511 18 ColbyJ

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Gerodontology 41 , 547554

DNA purification was carried out using SDSproteinase K digestion, phenol/chloroform extraction and ethanol precipitation