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Transmission electron microscopy The fixed organoids (described above) were rinsed and incubated overnight in 10% sucrose (in water) at 4 C, and the organoids were dehydrated in graded alcohols (50%, 75%, 96%, 100%), cleared in propylene oxide and embedded in a mixture of Epon 812 and Durcupan (Sigma

The transformation of HCy into cysteine is catalyzed by the enzymes CBS and CSE as part of the trans- sulfuration pathway (Figure 2)

Source: Park, Kyoung-Ha, and Woo Jung Park

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