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After batch normalization of peak intensity, a multivariate analysis was carried out using principal component analysis (PCA), partial least squares discriminant analysis (PLSDA), and orthogonal projections to latent structures discriminant analysis (OPLSDA) to identify differences between the Zn group and the control [20]

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Its initiation depends on intracellular iron overload, which serves as the key biochemical basis and trigger, ultimately leading to extensive membrane damage

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