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Silymarin has been shown to reduce liver injury and is used to treat alcoholic liver disease, acute and chronic viral hepatitis, and toxin-induced liver diseases

Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma, IPVH00010), blocked for 1 h using 5% BSA in TBS0.5% Tween-20 and stained overnight with primary rabbit-anti-GCLC (Sigma-Aldrich, HPA036359) or affinity-purified rabbit-anti-GGT1 (GGT129, as previously described 63 ) 1:1,000 in BSA 2.5% overnight at 4 C

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Gribble FM, Williams L, Simpson AK, Reimann F