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N Engl J Med 377:12281239 Lee YS, Jun HS (2016) Anti-Inflammatory Effects of GLP-1-based therapies beyond glucose control

Insulin Assay Islets from NOD scid or C57BL/6 mice were handpicked and seeded in to a U-bottom 96 well plate (10 islets per well) containing either 11 mM glucose, but no Ca 2+ , alone or with exendin-4 (Sigma-Aldrich) added at the indicated concentrations