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Low blood sugar (hyp oglycemia)
For semi-quantitative RT-PCR analyses, a limited number of cycles were conducted with a 1:5 diluted cDNA template, removing samples at the indicated cycle (72C step) and transfer to another thermocycler for completing the final elongation step at 72C for 5 min (and then kept on ice)
This observation was in agreement with several other studies which found that methane production was seen at lower levels in IBS-D and higher levels in IBS-C (Jahng et al., 2012
Gastroenterology & Hepatology, 19(3), 140
The amino acid sequence and the mass measured by MS are the only binding signals of identity the trade name alone is not enough to confirm which of the two hexapeptides is in the vial
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