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10.1186/s12986-015-0051-0 18

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Briefly, for SNAP/FLAG-hGLP-1R gold labelling, INS-1 832/3 SNAP/FLAG-hGLP-1R cells grown on Thermanox coverslips (Agar Scientific), or mouse islets transduced with SNAP/FLAG-hGLP-1R adenoviruses, were labelled with 2 M cleavable SNAP-Surface biotin probe (kind gift of Dr Ivan Corra Jr, New England Biolabs) in full media, followed by incubation with 5 g/mL NaN 3 -free Alexa Fluor 488 Streptavidin, 5 or 10 nm colloidal gold conjugate (Thermo Fisher Scientific) to label SNAP/FLAG-hGLP-1R prior to stimulation with 100 nM exendin-4, fixation and processing for conventional TEM

So few patients took it daily that a 30-day injectable form was invented

The oral tirzepatide guide covers the sublingual format of this alternative in detail