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Gene sets used were obtained from built-in Gene Ontology or curated databases v7.5 (Broad Institute) or described previously 78

Aging is associated with a reduced capacity to synthesize vitamin D in the skin upon sun exposure (49)

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The frozen sections were rehydrated with phosphate-buffered saline (PBS) and then blocked with PBS containing 1% normal mouse serum, 1% bovine serum albumin, and 0.3% Triton X-100, followed by staining with directly conjugated antibodies for 24 h at room temperature in a dark, humidified chamber