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Khawar IA, Kim JH, Kuh H-J (2015) Improving drug delivery to solid tumors: priming the tumor microenvironment

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The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M agonist, 0.01% (w/v) MNG and 0.006% (w/v) CHS to separate complex from contaminants