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These were produced by chronic exposure of INS-1 cells to 0.4 g/mL ethidium bromide in medium supplemented with sodium pyruvate and uridine, which are required to compensate for impaired mitochondrial metabolism

Under normal conditions, an enzyme system (NADPH-dependent methemoglobin reductase) keeps methemoglobin levels low, but if this system is overwhelmed (by oxidant drugs or toxins), MB administered intravenously acts as an artificial electron carrier

All these processes lead our cells to produce residual substances-or free radicals

65,66 Notably, molecular changes exhibited by non-proliferative reactive astrocytes can be fully reversible over time after single exposures for example to neuroinflammation caused by LPS

From fighting free radicals to boosting your immune system