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These Checklists list the requirements for completing the TUE Application Form, including the medical evidence that should be submitted along with it

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If reticulocytes have not increased after treatment or if reticulocyte counts do not continue at least twice normal as long as the hematocrit is less than 35%, diagnosis or treatment should be reevaluated

402,403,404 Approximately 15% of the global population carries the thalassemia gene mutation

Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol