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Bacterial colonies were verified for successful insertion of G6PD by colony PCR and positive clones were sent for sequencing (Eurofins) before transfection into M14 and UACC 62 cells

FIGURE 5 Ferroptosis, a regulatory cell death mode, is characterized by iron-dependent and lipid peroxide (LPO) accumulation, proposed by 2+ , which can produce OH through the Fenton reaction, and then react with polyunsaturated fatty acids in the plasma membrane to produce ROS, resulting in iron-regulated death

Copper Chaperone ATOX1 Is Required for MAPK Signaling and Growth in BRAF Mutation-Positive Melanoma

Summary of Costs and Benefits In section XXVI