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ELISA for circulating testosterone, LH, and IGF1 levels Total serum testosterone (T), IGF1, and LH concentrations of rats were measured on PNDs 24 and 31 by ELISA using testosterone and IGF1 ELISA kits (Demeditec Diagnostics, Kiel-Wellsee, Germany) and LH kit (Shibayagi, Shibukawa, Japan) according to the manufacturers instructions

Gouju J, Legeay S

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