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Subsequently, cells were centrifuged at 4 C, 4,000 g for 15 min, then resuspended in cold buffer A (20 mM HEPES-Na + , pH 8.0, 500 mM NaCl, 1 mM TCEP) and sonicated three times for 15 min each

Our results are supported by prior studies that highlighted the cardioprotective effect of ROSU through activating the SIRT1/FOXO3a pathway (Khayatan et al

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Dworski R: Oxidant stress in asthma