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In fact, mammalian fast-twitch (i.e., type II) muscle fibers exposed to GSH and reactant to induce S-glutathionylation showed an increase in their sensitivity to Ca 2+ , which was abrogated by the reducing agent dithiothreitol (DTT), indicating not only modification of protein thiols, but also that glutathionylation of skeletal muscle proteins can modulate Ca 2+ sensitivity [59]