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Each simulation protocol consisted of an initial 50 ns conventional MD (cMD) run to collect potential energy statistics for determining the GaMD acceleration parameters, followed by a 50 ns GaMD equilibration after applying the boost potential

We used a palbociclib dose of 25 mg/kg that had a moderate effect on MCF-7 growth but led to a significant reduction of tumour size in MCF-7 GPX4 null xenografts compared to those treated with vehicle (Fig