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IC 50 values were calculated using the AAT Bioquest calculator ( Vesicle preparation Vesicles were prepared as described 26

Additionally, c-fos (a neuronal activation indicator) was significantly enhanced in the arcuate nucleus (ARC) within 23 hours after tofogliflozin treatment, suggesting that tofogliflozin could permeate these regions and bind to receptors on the ARC

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