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Age-associated sperm DNA methylation alterations: possible implications in offspring disease susceptibility

Corresponding changes were observed at the phylum level (Figure S2D), where volcano plot analysis revealed marked alterations between groups, including pronounced upregulation of Pseudomonadota and Bacteroidota in the hCiPSCs-treated group, whereas Actinomycetota, Deferribacterota, and Bacillota were significantly depleted

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