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Hydrogen sulfide ameliorates cognitive dysfunction in streptozotocin-induced diabetic rats: involving suppression in hippocampal endoplasmic reticulum stress

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Ask the animal owners to describe the animal's medical history before applying the injection

In vivo chemogenetic manipulations For chemogenetic experiments in the DMV, rAAV-Ef1-DIO-hM3D(Gq)- mCherry-WPRE-pA (AAV-DIO-hM3Dq-mCherry, AAV2/9, 5.00 10 12 vg/mL) or rAAV-Ef1-DIO-hM4D(Gi)-mCherry-WPREs (AAV-DIO-hM4Di-mCherry, AAV2/9, 2.25 10 12 vg/mL) was injected into the DMV of C57 mice

Taking a vial straight from the freezer or fridge can cause condensation to form inside, which is not ideal