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RNA extraction, cDNA synthesis and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from cells using a TRI Reagent kit (Sigma) according to the manufacturers instructions

In cases where the GSH peak was undetectable, a value (the lowest measured GSH/Cr ratio value across all subjects and scans, divided by 2) was assigned

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J Pathol 217(4):571580 Shim WS, Ho IA, Wong PE (2007) Angiopoietin: a TIE(d) balance in tumor angiogenesis

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