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Description

In biochemical assays, an SCF E3 ligase complex containing Fbxo40 directly ubiquitinates IRS1, and this activity is enhanced by increased tyrosine phosphorylation of IRS1

The impact on other biological parameters was minimal

2.2 Production and purification of carnitine monooxygenase The individual subunits CntA and CntB of carnitine monooxygenase were produced and purified as described elsewhere (Massmig et al., 2020)