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Images were taken using a 10 objective with an excitation wavelength of 488 nm using 2.55% laser power with a frame time of 7.75 s, displaying a quarter of an optical slice of a 3-d post-injection oocyte

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"At least a 50% reduction in alcohol," the clinician said

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Meanwhile, the content of total NO was analyzed through determining nitrate and nitrite with the Total Nitric Oxide assay kit (Beyotime, Haimen, China) according to the manufacturers protocols