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Method Forty-two healthy older adults aged 5570 years were randomized to receive either a novel L-Carnitine (1500 mg), L-leucine (2000 mg), creatine (3000 mg), Vitamin D3 (10 g) (L-Carnitine-combination) product ( n = 14), L-Carnitine (1500 mg) ( n = 14), or a placebo ( n = 14) for eight weeks

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Different concentrations of ligand in stimulation buffer were added and the stimulation lasted for 40 min at RT