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The ROS generation was detected with a Leica fluorescence microscope (Leica DM6000B) using Leica LAS X software, and quantitative analysis was performed with ImageJ software (version 1.60

Inhibition of mitochondrial fission by reducing the phosphorylation of Drp1 and increasing mitochondrial autophagy and biosynthesis via overexpression of PTEN-induced kinase (PINK), parkinson juvenile disease protein 2 (parkin), mitochondrial transcription factor A (TFAM), and peroxisome proliferator-activated receptor gamma coactivator (PGC)-1 are ASX underlying mechanisms [31]

doi:10.1007/s00125-024-06144-1

These studies were the first to highlight the correlation between improvements in glycemia and reductions in plasma glucagon in diabetic subjects treated with a DPP4 inhibitor (169)