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Once cells reached 7585% confluence, they were treated with different concentrations of C3G (20, 40, and 80 g/mL) for 24 h

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The injection technique significantly impacts dose delivery

Or maybe you can either potentially also include some other hormetic stressors, things like sauna exposure or other types of hormesis as well

Our structure identified an unpredicted non-peptide agonist-binding pocket in which reorganization of extracellular loop 3 and transmembrane helices 6 and 7 manifests independently of direct ligand interaction within the deep transmembrane domain pocket